===== Usage ===== To use ``outrigger``, you must perform at least steps 1 and 3: 1. ``outrigger index`` to read all your junctions in and create a database of alternatively spliced exons. 2. ``outrigger validate`` to filter your splicing events for only those with valid introns. (optional) 3. ``outrigger psi`` to calculate percent spliced in on your splicing events. |OutriggerOverview| .. |OutriggerOverview| image:: _static/outrigger_overview-300ppi.png :target: _static/outrigger_overview-300ppi.png Each of these commands deserves its own page of explanation, so look at the links below. Contents: .. toctree:: :maxdepth: 2 subcommands/outrigger_index.rst subcommands/outrigger_validate.rst subcommands/outrigger_psi.rst